Assays Development Services

Assays play a key role in target validation, screening, selectivity profiling, and lead optimization. SBD provides cost-saving, high quality and reliable in vitro assay development, enzyme activity assays, cell-based assays, high throughput screening, and biophysical measurement of protein-protein and protein-small molecule interactions. Our professional scientists, with expertise in a variety of assay types, formats, and instruments are devoted to helping you accelerate drug development.

Assay Services

  • Assay development and optimization
  • Binding affinity measurements (proteins, compounds, nucleic acids)
  • Enzyme activity assays & kinetics
  • Dose-response activation or inhibition assays (IC50/EC50)
  • Cell-based and cell-free assays
Features
  • Standard or customized flexible service
  • Single sample, medium throughput or high throughput assay formats
  • Professional scientists with decades of experiences
  • Efficient and cost-saving experimental design
  • Reliable and reproducible results

Cell-based assays

SBD offers a broad range of cell-based assays to identify and quantitate biological activity of small molecules and proteins that regulate cell proliferation, apoptosis or cytotoxic cell death. We also develop custom cell-based assays to characterize the efficacy and safety of your compounds and biologics. Our experienced cell biologists will develop and implement the cell-based assays you need to quickly advance your drug discovery portfolio.

Enzyme activity assays

SBD provides various enzyme activity assays using spectrophotometry or fluorometry for kinases, phosphatases, deacetylase and other enzymes. Our enzyme activity assay services include novel assay development, kinetic parameter evaluation, dose-response assays and IC50 determination. Our experienced scientists and advanced facilities ensure fast, accurate and reproducible assay results.

Differential Scanning Fluorimetry (DSF) / Thermal Shift Assay

DSF is a thermal shift assay used to measure protein stability and protein-small molecule interactions. As the temperature is increased, non-covalent bonds are disrupted, exposing progressively more hydrophobic residues that interact with fluorescent dyes. The temperature at the transition midpoint during the protein unfolded process (Tm) is a measure of protein stability. Tm shifts correlate with protein binding interactions. With our experienced assay scientists and high throughput screening assay formats, SBD will help you find promising hits quickly.

Isothermal titration calorimetry (ITC)

ITC is a robust biophysical technique used to measure thermodynamic binding parameters (stoichiometry, enthalpy and entropy) in a single experiment. At SBD, we use thermal shift assay as a primary screen, and validate the strongest binders accurately using ITC. ITC measurements can be used to prioritize compounds for co-crystallization and rational drug design. SBD’s precision biophysical analysis using the MicroCal Auto-iTC200 is a powerful tool to efficiently guide compound optimization in drug discovery.